The REPELATION POTENCY OF N-HEXANE EXTRACT OF BASIL LEAF AND STEM (OCIMUM BASILICUM L.) AGAINST CULEX QUINQUEFASCIATUS

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Published Dec 25, 2021
https://doi.org/10.55116/IJIM.V1I1.24
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Vol. 2 No. 2 (2021): International Journal Islamic Medicine

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Indri Ramayanti Ramayanti
Universitas Muhammadiyah Palembang
Miranti Dwi Hartanti
Universitas Muhammadiyah Palembang
Reynaldi Aulia Rahman
Universitas Muhammadiyah Palembang

Abstract

The Culex quinquefasciatus mosquito is the main vector of filariasis and a popular effort to control it in society is by using chemical materials, but they cause toxic effects in humans, so it is necessary to use a repelant from natural ingredients, one of which is basil containing essential oils and proven to be effective as a repelant against mosquito. The objective of this research was to determine repelant power of n-hexane extract of basil leaves and stems against the Culex quinquefasciatus. This study was a laboratory experimental study using a completely randomized design, with 3 replications at 7 time intervals. The extract concentrations were 15%, 25%, 35 % and ethanol negative control 96%, the positive control repelant X containing 13% DEET. The results showed that the n-hexane extract of basil leaves and stems for 6 hours at a concentration of 15% was able to resist 75% of mosquito, a concentration of 25% was able to resist as much as 85.05%, and a concentration of 35% was able to resist as much as 92.51%,  the one-way ANOVA analysis showed that the value of p = 0.000 (p<0,05), meaning that there was a difference in the number of Culex quinquefasciatus perching on the black cloth at various concentrations of n-hexane extracts of leaves and stems of basil, while the probit analysis showed that the effective extract concentration at 90% was 31.52%. The extract of n-hexane leaves and basil stems was effective as a repelant against the Culex quinquefasciatus.

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How to Cite
1.
Ramayanti IR, Dwi Hartanti M, Aulia Rahman R. The REPELATION POTENCY OF N-HEXANE EXTRACT OF BASIL LEAF AND STEM (OCIMUM BASILICUM L.) AGAINST CULEX QUINQUEFASCIATUS. International J. of Islamic and Complementary Medicine [Internet]. 2021 Dec. 25 [cited 2024 Nov. 21];2(2):71-80. Available from: https://islamicmedicine.or.id/index.php/ijim/article/view/24

References

  1. Ahmed, M. et al. (2020) ‘Insecticidal activity and biochemical composition of Citrullus colocynthis, Cannabis indica and Artemisia argyi extracts against cabbage aphid (Brevicoryne brassicae L.)’, Scientific Reports, 10(1), pp. 1–10. doi: 10.1038/s41598-019-57092-5.
  2. Amoabeng, B. W., Johnson, A. C. and Gurr, G. M. (2019) ‘Natural enemy enhancement and botanical insecticide source: a review of dual use companion plants’, Applied Entomology and Zoology, 54(1), pp. 1–19. doi: 10.1007/s13355-018-00602-0.
  3. Asbahani, A. El et al. (2015) ‘Essential oils: From extraction to encapsulation’, International Journal of Pharmaceutics, 483(1), pp. 220–243. doi: 10.1016/j.ijpharm.2014.12.069.
  4. Bohbot, J. D. et al. (2011) ‘Conservation of indole responsive odorant receptors in mosquitoes reveals an ancient olfactory trait’, Chemical senses. 2010/10/18. Oxford University Press, 36(2), pp. 149–160. doi: 10.1093%2Fchemse%2Fbjq105.
  5. Brito, N. F., Moreira, M. F. and Melo, A. C. A. (2016) ‘A look inside odorant-binding proteins in insect chemoreception’, Journal of Insect Physiology, 95, pp. 51–65. doi: 10.1016/j.jinsphys.2016.09.008.
  6. Campos, E. V. R. et al. (2019) ‘Use of botanical insecticides for sustainable agriculture: Future perspectives’, Ecological Indicators, 105, pp. 483–495. doi: 10.1016/j.ecolind.2018.04.038.
  7. Chaudhary, A. et al. (2020) ‘Phytochemical and antioxidant profiling of Ocimum sanctum’, Journal of Food Science and Technology, 57(10), pp. 3852–3863.
  8. Chellappandian, M. et al. (2018) ‘Botanical essential oils and uses as mosquitocides and repellents against dengue’, Environment International, 113, pp. 214–230. doi: 10.1016/j.envint.2017.12.038.
  9. Deletre, E. et al. (2016) ‘Prospects for repellent in pest control: current developments and future challenges’, Chemoecology, 26(4), pp. 127–142. doi: 10.1007/s00049-016-0214-0.
  10. Demiray, H. et al. (2019) ‘Chemical composition of the essential oil and n-hexane extract of Stachys tmolea subsp. Tmolea Boiss., an endemic species of Turkey, and their mosquitocidal activity against dengue vector Aesdes aegypti’, Saudi Pharmaceutical Journal. Elsevier, 27(6), pp. 877–881. doi: 10.1016/j.jsps.2019.05.009.
  11. Diop, A. et al. (2016) ‘Monitoring survey of the use patterns and pesticide residues on vegetables in the Niayes zone, Senegal’, Chemosphere, 144, pp. 1715–1721.
  12. Dirar, A. I. et al. (2019) ‘Effects of extraction solvents on total phenolic and flavonoid contents and biological activities of extracts from Sudanese medicinal plants’, South African Journal of Botany. South African Association of Botanists, 120, pp. 261–267. doi: 10.1016/j.sajb.2018.07.003.
  13. Farzaei, M. H. et al. (2017) ‘Pharmacological activity of Mentha longifolia and its phytoconstituents’, Journal of Traditional Chinese Medicine, 37(5), pp. 710–720.
  14. IAEA (2017) ‘Guidelines for Standardised Mass Rearing of Anopheles Mosquitoes’, in. Vienna: Food and Agriculture Organization of the United Nations International Atomic Energy Agency Vienna, p. 44.
  15. Iskandar, B. S. et al. (2020) ‘Various medicinal plants traded in the village market of karangwangi village, southern cianjur, west java, indonesia’, Biodiversitas, 21(9), pp. 4440–4456. doi: 10.13057/biodiv/d210963 Various.
  16. Isman, M. B. (2017) ‘Bridging the gap: Moving botanical insecticides from the laboratory to the farm’, Industrial Crops and Products. Elsevier, 110(April), pp. 10–14. doi: 10.1016/j.indcrop.2017.07.012.
  17. Legeay, S. et al. (2018) ‘Unusual modes of action of the repellent DEET in insects highlight some human side effects’, European Journal of Pharmacology, 825, pp. 92–98. doi: 10.1016/j.ejphar.2018.02.033.
  18. Lombardo, F. et al. (2017) ‘Deciphering the olfactory repertoire of the tiger mosquito Aedes albopictus’, BMC Genomics. BMC Genomics, 18(1), pp. 1–23. doi: 10.1186/s12864-017-4144-1.
  19. Mierziak, J., Kostyn, K. and Kulma, A. (2014) ‘Flavonoids as important molecules of plant interactions with the environment’, Molecules, 19(10), pp. 16240–16265. doi: 10.3390/molecules191016240.
  20. Montell, C. and Zwiebel, L. J. (2016) ‘Mosquito Sensory Systems’, in Raikhel, A. S. (ed.) Progress in Mosquito Research. Science direct (Advances in Insect Physiology), pp. 293–328. doi: 10.1016/bs.aiip.2016.04.007.
  21. Ntalli, N. et al. (2019) ‘Plant secondary metabolites against arthropods of medical importance’, Phytochem Rev, 18(5), pp. 1255–1275. doi: 10.1007/s11101-019-09647-7.
  22. Ponsankar, A. et al. (2016) ‘Target and non-target toxicity of botanical insecticide derived from Couroupita guianensis L. flower against generalist herbivore, Spodoptera litura Fab. and an earthworm, Eisenia foetida Savigny’, Ecotoxicology and Environmental Safety. Elsevier, 133, pp. 260–270. doi: http://dx.doi.org/10.1016/j.ecoenv.2016.06.043 0147-6513/&.
  23. Sankhalkar, S. and Vernekar, V. (2016) ‘Quantitative and Qualitative Analysis of Phenolic and Flavonoid Content in Moringa oleifera Lam and Ocimum tenuiflorum L.’, Pharmacognosy research, 8(1), pp. 16–21. doi: 10.4103/0974-8490.171095.
  24. Silverio, M. R. S. et al. (2020) ‘Plant natural products for the control of Aedes aegypti: The main vector of important arboviruses’, Molecules, 25(15). doi: 10.3390/molecules25153484.
  25. Swale, D. R. et al. (2014) ‘Neurotoxicity and mode of action of N, N-Diethyl-Meta-Toluamide (DEET)’, PLOS ONE. Public Library of Science, 9(8), pp. 1–11. doi: 10.1371/journal.pone.0103713.
  26. Tian, H., Fürstenberg, A. and Huber, T. (2017) ‘Labeling and single-molecule methods to monitor G protein-coupled receptor dynamics’, Chemical Reviews, 117(1), pp. 186–245. doi: 10.1021/acs.chemrev.6b00084.
  27. Tzotzos, G., Iley, J. N. and Moore, E. A. (2018) ‘New insights on repellent recognition by Anopheles gambiae odorant-binding protein 1’, PLOS ONE. Public Library of Science, 13(4), pp. 1–23. doi: 10.1371/journal.pone.0194724.
  28. Vinauger, C. et al. (2018) ‘Modulation of host learning in Aedes aegypti mosquitoes’, Current Biology, 28(3), pp. 333-344.e8. doi: 10.1016%2Fj.cub.2017.12.015.
  29. WHO (2009) Guidelines for Efficacy Testing of Mosquito Repellents for Human Skin. Control of Neglected Tropical Diseases WHO Pesticide Evaluation Scheme.
  30. Zahran, E. M. et al. (2020) ‘Diversity, phytochemical and medicinal potential of the genus Ocimum L. (Lamiaceae)’, Phytochemistry Reviews, 19(4), pp. 907–953.
  31. Zaniol, F. et al. (2020) ‘Comparative larvicidal effect of Pterodon spp. extracts obtained by different extraction methods’, The Journal of Supercritical Fluids, 166. doi: 10.1016/j.supflu.2020.104993.